This method estimates prevalence and confidence limits for variable pool sizes and assumed 100% test sensitivity and specificity. This method should be used if you can be confident that the sensitivity and specificity of the test are both close to 100%, and if pool sizes vary.

For this analysis, it was assumed that samples from 210 individual fruit bats were aggregated into 40 pools of 5 samples each with 10 samples tested individually, that 20 pools and 2 individual samples produced a positive test result and that the test sensitivity and specificity were both 100%. Input values and results for this analysis are summarised in the table below.

Input values: | ||
---|---|---|

Confidence level | 0.95 | |

Pool size | Number of pools tested |
Number of pools positive |

5 | 40 | 20 |

1 | 10 | 2 |

Results: | ||

Estimated Prevalence | 0.1339 | |

2.5 percentile | 0.0871 | |

97.5 percentile | 0.1930 |

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