This program calculates the approximate numbers of pools required for given values for pool
size, estimated prevalence and desired confidence and precision of the estimate, assuming fixed pool sizes and a
test with 100% sensitivity and specificity. The required number of pools (m) to estimate pi
with a perfect test and fixed pool size (k) can be calculated using the following equation, where
e is the desired precision (allowable error) of the estimate and Z is the standard normal variate
for the desired level of confidence. See the User Guide or
Worlund & Taylor (1983) for more details.
See demonstration analysis
m(perfect test) = (Z(1  pi)/(ek))^2((1  pi)^k  1)
For fixed pool size and perfect tests, the optimum value of m can be calculated that minimises
the variance of the estimated prevalence and consequently minimises the number of pools requiring
testing to achieve the desired confidence and precision. This optimum value for m depends on the
prevalence and is approximately 1.6/pi. This equates to the pool size which results in an expected
number of 1.6 infected individuals per pool. See the User
Guide or Sacks et al. (1989) for more details.
In practice, it is advisable to select a smaller pool size and test a larger number of pools to
minimise potential bias in the result.
Input values
Required inputs are the estimated true prevalence, the desired level of precision (or acceptable
error) and the desired level of confidence. For example, you might wish to estimate the prevalence
where the true value is assumed to be about 0.01 (1%), and you wish to have 95% (0.95) confidence
that the true value is within +/ 0.005 (0.5%) of your estimate. You can also input a suggested pool
size if desired, and the program will calculate the corresponding number of pools to be tested for
that pool size (in addition to predetermined pool sizes). Suggested pool size is ignored if it is zero.
Outputs
The program calculates the number of pools required for the inputscenario for a range of pool sizes
and presents the results in tabular and graphical formats. The optimum pool size and
corresponding number of pools are also calculated.
