Pooled Prevalence Calculator – Demonstration analyses

Variable pool size and perfect test

This method estimates prevalence and confidence limits for variable pool sizes and assumed 100% test sensitivity and specificity. This method should be used if you can be confident that the sensitivity and specificity of the test are both close to 100%, and if pool sizes vary. 

For this analysis, it was assumed that samples from 210 individual fruit bats were aggregated into 40 pools of 5 samples each with 10 samples tested individually, that 20 pools and 2 individual samples produced a positive test result and that the test sensitivity and specificity were both 100%. Input values and results for this analysis are summarised in the table below.

Variable pool size

 

Input values:

 

 

Confidence level:

0.95

 

Pool size

Number of pools tested

Number of pools positive

5

40

20

1

10

2

 

 

Results:

 

Est. Prevalence

0.1339

2.5 percentile

0.0871

97.5 percentile

0.1930

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