Pooled Prevalence Calculator – Demonstration analyses
Sample size calculation for fixed pool size and tests with uncertain sensitivity and specificity
This option can be used to calculate the number of pools that need to be tested for various values for pool size, estimated prevalence and desired confidence and precision of the estimate. It assumes fixed pool sizes and that the true values of both sensitivity and specificity are not known exactly and have been estimated in a limited number of samples.
For this analysis, sample size was calculated to provide 95%
confidence of estimating a true prevalence of 0.14 (14%) with a precision of
0.055 (5.5%). This is equivalent to the observed prevalence and precision when
162 samples from little red flying foxes in
Input 
Value 
0.14 

0.055 

0.95 

0.9 

1 

50 

10000 
Pool size 
Number of pools 
Total number of individual samples 
1 
183 
183 
2 
102 
204 
5 
59 
295 
10 
67 
670 
15 
238 
3570 